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This study was carried out on six buffalo bulls (Bubalus bubalis) for a one-year period. Four bulls selected had good and two bulls (No. 321 and 323) had poor semen quality. All bulls were aged from 6-10 years. Scrotal circumference (SC) and sperm characteristics of both bulls were lower than healthy bulls, while dead sperm percentage and total sperm abnormalities were high. Overall seminal plasma testosterone was lower in these bulls, while oestrogen was lower in bull 323 and higher in 321. Histopathological studies of testes of bull 323 showed a 100% loss of germinal epithelium (DGEL) in all three regions of the right testis; however, it was 89.96% in left testis. DGEL in bull 321 was 35.88 % in right and 31.70% in left testis, with higher DGEL in the ventral part in both testes. Total and lumen diameter was greater (P
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