الكاتب: Multiple References

Postpartum buffaloes (n = 23) not resuming or not showing observed estrus within 60 days after calving were classified as cyclical subestrus or anestrus based on palpable ovarian structures were divided into ovsynch-subestrus (n = 12) and ovsynch – anestrus (n = 11) groups. Ovsynch buffaloes were subjected to ovsynch protocol which consisted of 100 µg i.m. injection of GnRH on the day of start of synchronization (d 0), 25 mg i.m. injection of PGF2 a seven days later (d 7), another 100 µg i.m. injection of GnRH 48 h after PGF2 a (d 9) and timed insemination 16 to 18 h after second GnRH injection (d 10) for synchronization of ovulation. Blood samples were collected from all experimental animals on d -10 and d 0 for the estimation of progesterone. The estrus detection rates were 41.66 and 27.27 per cent in ovsynch-subestrus and ovsynch-anestrus respectively. Most buffaloes showed intermediate signs of estrus in ovsynch-subestrus group but buffaloes of ovsynchanestrus group showed predominantly weak estrus. The ovulatory response and first service conception rates were 83.33 and 33.33 and 54.54 and 0 per cent in ovsynch-subestrus and ovsynch-anestrus groups, respectively.